L8015-03F
Lymphotoxin beta Receptor (LTBR, LT-beta-R, Tumor Necrosis Factor C Receptor, Tumor Necrosis Factor Receptor Superfamily Member 3) (FITC)
Size
£639.00
- SKU:
- L8015-03F
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Lymphotoxin beta Receptor Human, IgG2a Fc Mouse, Fusion Protein (L8015-03B).
- Clone:
- 7H156
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Human Lymphotoxin beta receptor (LTbR, TNFSF3) is a member of the TNF receptor superfamily with similarilty to CD120a(TNFR1) and CD120b(TNFR2). It is a expressed mainly on non lymphoid tissues (1) and appears to have an important role in secondary lymphoid organ development. Suppression of LTbR signaling can alleviate autoimmunity or exasperate mycobacterial infection. Its ligands include Ltab2 and LIGHT. In mice, LTbR signaling is important for development and function of HEV, Dendritic cells, and Mast cells. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (1.6ul/5x10e5 cells) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Pre-incubated 5x10e5 cultured human U-937 cells with 20ul of 150ug/ml human IgG (to block non specific binding). 2. Incubate cells for 45 minutes on ice with 80ul of L8015-03F that has been previously diluted 1:50 (10ug/ml). 3. Washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.85 log10 fluorescent units when compared to a Mouse IgG1(FITC) negative control (I1904-75D) at a similar concentration. Binding was blocked when reagent was pre- incubated with 50ug/ml of recombinant LTbR-muIg (L8015-03B). Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice