L7715-05C

Ly-6A/E (Ly6A/E, Lymphocyte Antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Ly6a, Ly6, Ly-6A.2, Ly-6E.1, Stem Cell Antigen 1, Sca1, SCA-1, T-cell-activating Protein, TAP, Lymphocyte Activation Protein 6) (PE)

Size

£435.00

SKU:: L7715-05C
Purity:: Purified
Storage Conditions:: 4°C Do not freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: IL-2-dependent mouse T-cell line CTL-L
Clone:: D7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).
Extra Details:: Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes.1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.1, 8, 9 Studies with this antibody suggest that Ly-6A/E may be involved in B and T lymphocyte responses,9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.3, 13 Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry and In vitro lymphocyte activation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤0.2ug/10e6 cells Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice