L7715-05A

Ly-6A/E (Ly6A/E, Lymphocyte Antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Ly6a, Ly6, Ly-6A.2, Ly-6E.1, Stem Cell Antigen 1, Sca1, SCA-1, T-cell-activating Protein, TAP, Lymphocyte Activation Protein 6) (FITC)

Size

£499.00

SKU:: L7715-05A
Purity:: Purified
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rat
Immunogen:: IL-2-dependent mouse T-cell line CTL-L
Clone:: D7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with FITC.
Extra Details:: Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with this antibody suggest that Ly-6A/E may be involved in B and T lymphocyte responses and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation. Applications: Suitable for use in Flow cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry and In vitro lymphocyte activation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Shipping Conditions:: Blue Ice