L7715-05

Ly-6A/E (Ly6A/E, Lymphocyte Antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Ly6a, Ly6, Ly-6A.2, Ly-6E.1, Stem Cell Antigen 1, Sca1, SCA-1, T-cell-activating Protein, TAP, Lymphocyte Activation Protein 6)

Size

£406.00

SKU:: L7715-05
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: IL-2-dependent mouse T-cell line CTL-L
Clone:: D7
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins (1-3). It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes (4-6). In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes (1, 7-9). The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation (1, 8, 9). Studies with this antibody suggest that Ly-6A/E may be involved in B and T lymphocyte responses (9-12) and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation (3, 13). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry and In vitro lymphocyte activation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice