L5999-06A
Luciferase, photinus pyralis
Size
£639.00
- SKU:
- L5999-06A
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Luciferase expressed in E. coli.
- Clone:
- 3H2605 (LUC-Y)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required Immunohistochemistry: Frozen sections ELISA: 1:500-1:5000 Western Blot: 1:100. A band of ~55-60kD is detected using a sample of 1ug of purified luciferase from Photinus pyralis. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Hybridoma: Sp20 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice