L4502-21J
LRRK2 (Leucine-rich Repeat Kinase 2, RIP7, PARK8, Dardarin)
Size
£594.00
- SKU:
- L4502-21J
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000). [Swiss-Prot# Q5S006]
- Format:
- Supplied as a liquid in Tris-citrate-phosphate, pH 7-8, 0.09% sodium azide.
- Extra Details:
- This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2 ug/ml, A band is seen at ~284kD. There is a band at ~171 and 117kD, as well as several lower molecular weight, non-specific bands. Immunohistochemistry: 2.5-5.0ug/ml, (Paraffin): 2.5-5.0ug/ml Optimal dilution determined by the researcher. Positive Controls: Mouse brain lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice