L4500-03J

LRP, MVP (Lung Resistance-related Protein, Major Vault Protein, VAULT 1, VAULT1)

Size

£709.00

SKU:: L4500-03J
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human non-P-glycoprotein MDR tumor cell lines.
Clone:: MVP-37
Format:: Supplied as a liquid in 0.7% BSA, 0.09% sodium azide.
Extra Details:: MVP/LRP (p110) is strongly overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen, paraffin): 1:20-1:50 on 4% paraformaldehyde fixed cytospin preparations or frozen tissue sections (apply the following pretreatment: 10min 20mM glycine, pH 7.5, 10 min 6N guanidine hydrochloride in 50mM Tris-HCl, pH 7.5; Schroeijers et al., 2000). MVP-37 performs well on paraffin-embedded tissue sections. Western Blot (ECL): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.