L3185-03-HRP
LKB 1 (HRP)
Size
£1,070.00
- SKU:
- L3185-03-HRP
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein corresponding to residues 1-433 of human LKB1.
- Clone:
- 5H64
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: detected LKB1 in RIPA lysates from Rat2 cells. Rat2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with L3185-03 (1ug/ml). Proteins were visualized using a IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunocytochemistry: detected LKB1 in transfected Cos-7 cells fixed with 3-7% paraformaldehyde and permeabilized with 0.1% Triton X-100. Optimal dilutions to be determined by the researcher. *Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice