Livin (ML-IAP)

Size

£655.00

SKU:: L3180-02
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic 17-amino acid peptide near the C-terminus of human Livin.
Format:: Supplied as a liquid in PBS containing 0.02% sodium azide.
Extra Details:: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP (1,2). Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-a and Livin-b (1,3). Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6 (1). Livin has direct interaction with several caspases including caspase-3, -7, and Ð9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions. Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml. A band at 33kD (predicted MW) can be detected. A lower but much weaker ban at 30kD was detected in Raji cell lysate, which may represent the short form of Livin. Immunohistochemistry: 5ug/ml. Immunofluorescence: 20ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: Raji cell lysate; human small intestine tissue lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice