Liver Specific Transporter (LST-1) (Not for Export EU)

Size

£704.00

SKU:: L3176-07
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 17aa from human Liver Specific Transporter (LST-1) conjugated to KLH.
Format:: Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Extra Details:: Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Most recently, a novel liver-specific OAT, LST-1, has been cloned from human liver. It has also been detected in mouse and rat liver. No significant expression of LST-1 was detected in other tissues. Human LST-1 (691 aa, 12 TM) shares 42% homology with human OATP, rat oatp1-3, and OATK-1, and 35% with PGT. Phylogenetic analyses places LST-1 between oatp and PGT. LST-1 has wide substrate specificity (taurocholate, conjugated steroids, PGE2, Thromboxane B2, leukotrienes, and T3). Therefore, LST-1 may be more important for clearance of bile acids and organic anions in liver. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-50,000, Control peptide (cat # L3176-05) can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice