L2526-02

Lipopolysaccharide-induced CXC Chemokine (LIX, CXCL6) (Biotin)

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£479.00

SKU:: L2526-02
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to mouse LIX, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to a concentration of 0.05mg/ml.
Extra Details:: LIX is a 9.9kD chemokine, expressed by fibroblasts, that is induced by bacterial lipopolysaccharides and is chemotactic for neutrophils. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.25-1ug/ml using 100ul/well. in conjunction with L2526-01A as the capture antibody, the detection limit is 0.2-0.4ng/well of recombinant mouse LIX Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse LIX is 1.5-3ng/lane under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.