Lipocalin 2, NT (Lipocalin-2, Lcn2, 24p3, 25kD alpha-2-microglobulin-related Subunit of MMP-9, HNL, MSFI, Neutrophil Gelatinase-associated Lipocalin, NGAL, Oncogene 24p3, p25)

Size

£837.00

SKU:: L2499-15M
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to the mature chain of human LCN2 (KLH). Species sequence homology: mouse, bovine and rat.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: LCN2 (also known as Neurophil Gelatinase-Associated Lipocalin or NGAL) belongs to a superfamily of lipocalins, a collection of small, diversely functional, extracellular proteins. NGAL is secreted from neutrophil granules in three different forms; a monomer of 25 kDa, a disulfide-linked homodimer at 46 kDa, and a disulfide-linked heterodimer with MMP-9 at 135-kDa. It is widely known to induce development of kidney epithelia, to bind and traffic iron, and to act as a molecular signal under inflammatory conditions, such as Crohn’s Disease. Multiple carcinoma studies have also shown elevated expression levels of LCN2 in several different human tumors affecting such organs as: colon, lung, breast, ovaries and pancreas. Additionally, there has been great interest in this protein as a possible marker for: onset of Acute Kidney Injury following cardiac surgery, the progression of Chronic Kidney Disease (CKD), and the survival chances of patients with Chronic Heart Failure (CHF). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Westrn Blot: 5ug/ml detected LCN2 in 10ug of human kidney tissue lysate. Immunohistochemistry: 1:200 detected LCN2 in human spleen tissue. Optimal dilutions to be determined by the researcher. Positive Control: Human kidney tissue lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice