Lipin 2 (Lipin-2, LPIN2, KIAA0249)

Size

£754.00

SKU:: L2497-10A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 14aa peptide of mouse Lipin 2, near the N-Terminal. No significant sequence homology is seen with Lipin 1, Lipin 3 or other proteins. Species Sequence Homology: Human - 69%.
Format:: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Extra Details:: The actions of insulin culminate in changes in the phosphorylation state of a number of downstream targets including phosphatidylinositol 3-OH kinase (PI3-kinase). Activation of PI3-kinase is central to insulin-stimulated phosphorylation in fat cells. In adipocytes, PI3 is involved in activation of protein kinase B (PKB), glycogen synthase, transitional regulators, 4E-BP1, p70s6k and mTOR protein (mammalian target of rapamycin). The kinase activity of mTOR functions in nutrient sensing pathway that maintains a proper balance of amino acid availability, protein synthesis and cell growth. Most importantly, mTOR controls the phosphorylation of a newly discovered protein Lipin-1, required for normal adipose tissue development and metabolism. The mutation in Lipin-1 gene results in immature adipocytes and thus in fatty liver dystrophy (fld) phenotype in mice and in lipodystrophy, a group of rare human diseases. These phenotypes are characterized by a triglyceride-filled fatty liver, loss of body fat, hypertrigyceridemia, insulin resistance, increased susceptibility to atherosclerosis, reduced fertility, reduced plasma Leptin and a progressive neuropathy affecting peripheral nerves in adulthood. Lipin defines a family of nuclear proteins containing at least three members in human and mouse: Lipin-1, Lipin-2 and Lipin-3. All Lipin members contain a nuclear signal sequence, a highly conserved amino- (NLIP) and a carboxy-terminal (CLIP), domains. The human and mouse LPIN-2 genes have been mapped at chromosomes 18p and 17, respectively. The Lipin 2 sequences from mouse and human are ~90% identical. The overall amino acid sequence of mouse Lipin 2 (891aa) is ~48% identical to mouse Lipin 1(891aa) and Lipin 3 (848aa). Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml; Control peptide (Cat. # L2497-10B) can be used to coat ELISA plates at 1ug/ml. Western Blot: 1-10ug/ml using ECL. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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