L2497-01E
Lipin 1 (LPIN1, Phosphatidate Phosphatase LPIN1, KIAA0188)
Size
£594.00
- SKU:
- L2497-01E
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa300-400 from the internal region of human LPIN1.
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide, 30% glycerol.
- Extra Details:
- LPIN1 (phosphatidate phosphatase LPIN1) is expressed most abundantly in adipocytes and skeletal muscles wherein it play two major roles (i) in the cytoplasm, LPIN1 acts as a phosphatidate phosphatase 1 (PAP1) enzyme converting phosphatidic acid to diacylglycerol which is the precursor of both triglycerides and phospholipids (ii) in the nucleus, LPIN1 functions as a transcriptional co-activator through interaction with transcription factors which regulate the expression of genes involved in energy pathways. It can interact with MEF2C and LPIN1 interction with PPARA as well as PPARGC1A leads to a complex formation that modulates gene transcription. Three LPIN-1 protein isoforms are generated by alternative splicing, specifically involved in either PAP1 activity, transcriptional function or lipid droplets biogenesis. Defects in LPIN1 are a cause of autosomal recessive acute recurrent myoglobinuria (ARARM).. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml, band at ~97kD Immunohistochemistry (paraffin): 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice