L2497-01A

Lipin 1

Size

£754.00

SKU:: L2497-01A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic 16aa peptide sequence (aa111-126) at the N-terminus of mouse Lipin 1 (KLH coupled).
Format:: Supplied as a liquid in PBS, pH7.5, 0.1% BSA, 40% glycerol.
Extra Details:: The actions of insulin culminate in changes in the phosphorylation state of a number of downstream targets including phosphatidylinositol 3-OH kinase (PI3-kinase). Activation of PI3-kinase is central to insulin-stimulated phosphorylation in fat cells. In adipocytes, PI3 is involved in activation of protein kinase B (PKB), glycogen synthase, transitional regulators, 4E-BP1, p70s6k and mTOR protein (mammalian target of rapamycin). Kinase activity of mTOR functions in nutrient sensing pathway that maintains a proper balance of aa availability, protein synthesis and cell growth. Most importantly, mTOR controls the phosphorylation of a newly discovered protein Lipin-1, required for normal adipose tissue development and metabolism. The mutation in Lipin-1 gene results in immature adipocytes and thus in fatty liver dystrophy (fld) phenotype in mice and in lipodystrophy, a group of rare human diseases. Besides Lipin-1, there are at least 2 more nuclear proteins (Lipin-2 and Lipin-3) in human and mouse lipin families All lipin members contain a nuclear signal sequence, a highly conserved amino-(NLP) domain and a carboxy-terminal (CLIP) domain. The human and mouse LPIN-1 genes have been mapped at chromosomes 2p21 and 12, respectively. A region (POMC) on this same chromosome also encodes pro-opiomelanocortin, a locus linked to total adiposity and the levels of plasma protein Leptin. The mouse Lipin-1 exists as isoform 1(891aa, missing 241-273 aa) and isoform 2 or isoform b (924 aa). It is a 140kD protein with many potential phosphorylated sites. The overall aa sequence of mouse Lipin-1 is over 46 and 49% identical to mouse Lipin-2 and Lipin-3, respectively. The seqs of human and mouse Lipin-1 are over 88% identical. Lipin-1 mRNA is prominently expressed in adipose tissue, skeletal muscle and testis with lower expression in kidney, lung, brain and liver. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL. Will recognize ~140kD mouse Lipin-1 under non-reducing conditions. It is not clear if isoform 2 is also recognized with this antibody. ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: L2497-01B Lipin 1, Mouse Control Peptide Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice