L2028-65
Leukocyte Immunoglobulin-like Receptor 8 (LIR8, LILRB5, CD85c)
Size
£825.00
- SKU:
- L2028-65
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg18-His456 from human LIR 8, expressed in NS0 cells.
- Clone:
- 7H202
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human LIR-8 (leukocyte immunoglobulin-like receptor 8; also CD85c and LILRB5) is a 567 amino acid (aa) member of the LIR family of receptors. It is a type I transmembrane protein that is expressed on NK cells. LIR8 has a 435 aa extracellular domain with four C2-type Ig-like domains (aa 24-458), and a 111 aa cytoplasmic tail that contains two ITIM motifs. Phosphorylation of Tyr in an ITIM motif recruits SHP-1, which is subsequently activated and impacts related signaling pathways. The ligand for, and function of, LIR8 are unreported. LIR-8 has two potential splice forms. One shows a 53 aa cytoplasmic insert between Arg542 and Ala543. The second shows a deletion of 100 aa between Gly119 and Gly219 that impacts the second C2-type Ig-like domain. There is no rodent ortholog. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human LILRB5/CD85c/LIR-8 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice