L2028-59
LILRA5 (Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5, CD85 Antigen-like Family Member F, Immunoglobulin-like Transcript 11, ILT-11, Leukocyte Immunoglobulin-like Receptor 9, LIR-9, CD85f, ILT11, LILRB7, LIR9) (PE)
Size
£780.00
- SKU:
- L2028-59
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly42-Arg268 from human LILRA5 expressed in CHO cell line.
- Clone:
- 12K126
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- ILT11 is a 39-40kD Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of pro-inflammatory cytokines, such as TNF-A and IL-1B. Mature human ILT11 is a 258aa type I transmembrane glycoprotein. It has a 227aa extracellular domain aa42-268 that contains two C2 type Ig-like domains aa51-136 and 142-230, and a 10aa cytoplasmic tail. ILT11 has three potential splice forms. One is a 35kD soluble form of the molecule described above that shows a 27aa substitution for aa239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa41-268, human ILT11 shares 58% aa identity with mouse LIR-9. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 human peripheral blood monocytes Optimal dilutions to be determined by the researcher. Emission: 565-605nm Excitation: 488nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice