L2020-75
Leucine Rich Repeat and Ig Domain Containing 2 (LINGO2, LRRN6C, LERN3)
Size
£825.00
- SKU:
- L2020-75
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Cys28-Leu542 of human LINGO2 expressed in NS0 cells. Species Sequence Homology: canine, 99%; mouse, 98%
- Clone:
- 7H175
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human LINGO2 is a type I transmembrane protein in the neuronal leucine-rich repeat family. These proteins have a signal peptide, 12 extracellular leucine-rich repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail. An alternate start site may exist at Met148 of the precursor. Human LINGO2 is a highly conserved, 606 amino acid protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO2, respectively. LINGO2 presumably functions outside the CNS with little involvement by p75/NgR1. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml SH-SY5Y human neuroblastoma cell line. Flow Cytometry: 2.5ug/10e6 cells. A549 human lung carcinoma cell line and HEK293 human embryonic kidney cell line either transfected with human LINGO2 and eGFP Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice