Leptin Receptor Gene Related Protein (OBGRP)

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£754.00

SKU:: L1671-24
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 15aa within the first putative intracellular domain of human OB-RGRP, conjugated to KLH. Species Sequence Homology: human LEPROTL1, 53%
Format:: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Extra Details:: Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (167 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-50,000; 10-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Control Peptide: L1671-24B: Leptin Receptor Gene Related Protein, Human Control Peptide Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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