L1670-10

Leptin, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor)

Size

£429.00

SKU:: L1670-10
Molecular Weight:: 16.2
Purity:: ≥ 98% (SDS-PAGE and HPLC). Endotoxin: ≤1EU/μg (0.1ng/ug).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 0.1% TFA. Reconstitute with sterile ddH2O to a concentration of ~1mg/ml. This solution can then be diluted into other aqueous solutions.
Extra Details:: Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant mouse leptin is a 16.2kD protein containing 147 amino acid residues. Biological Activity: Determined by an-in vivo-assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5μg leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed compared to saline-treated controls. Amino Acid Sequence: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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