L1670-08G

Leptin, aa92-145 (Ob, Obese Protein, Obesity Factor)

Size

£448.00

SKU:: L1670-08G
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human leptin (92-145) cyclized
Clone:: 2F9-F4
Format:: Supplied as a liquid.
Extra Details:: Applications: Immunofluorescent Staining of Cells This monoclonal antibody has been found to stain specifically human adipocytes at a 1:25 dilution. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum, reacted for 60 minutes with the diluted mouse monoclonal antibody, and then with FITC-conjugated goat anti-mouse IgM. The immunofluorescent staining pattern was observed using epifluorescence microscopy. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.