L1670-07D

Leptin (Ob, Obese Protein, Obesity Factor)

Size

£767.00

SKU:: L1670-07D
Purity:: Affinity purified over the control-peptide Sepharose
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified, human recombinant leptin
Clone:: 3H31
Format:: Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
Extra Details:: Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using neat serum) with Chemiluminescence technique. The obese protein is predicted to be secretory protein with a molecular weight of ~ 16kD (refs. 1, 2). ELISA: 1:10,000-50,000; 10-100ng of Leptin protein/well). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.