L1669-01B1
Leptin (Ob, Obese Protein, Obesity Factor)
Size
£832.00
- SKU:
- L1669-01B1
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to human leptin.
- Clone:
- 11C130
- Format:
- Supplied as a liquid in PBS, pH7.4, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. F/P ratio: ~10-20:1.
- Extra Details:
- Human Leptin is an 167aa residue, ~16kD, adipocyte derived, secreted protein. Human Leptin was cloned, expressed in E. coli and purified. The purity of Leptin is greater than 95% as determined by SDS-PAGE. The identity of protein has been confirmed by N-terminal sequence analyses. Endotoxin level in the final preparation is less than 1pg/ug of murine Leptin. Mouse leptin is about 85% homologous with human Leptin. Purified human Leptin has been shown to be biologically active in reducing body weight, food consumption, and plasma glucose levels in ob/ob and NZO mice. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:30,000 (10-100ng of Leptin protein/well) Western Blot: 1:2000-1:10,000 (chemiluminescence) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice