L1669-01B
Leptin (Ob, Obese Protein, Obesity Factor)
Size
£927.00
- SKU:
- L1669-01B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to human leptin.
- Clone:
- 11C130
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- Human Leptin is an 167aa residue, ~16kD, adipocyte derived, secreted protein. Human Leptin was cloned, expressed in E. coli and purified. The purity of Leptin is greater than 95% as determined by SDS-PAGE. The identity of protein has been confirmed by N-terminal sequence analyses. Endotoxin level in the final preparation is less than 1pg/ug of murine Leptin. Mouse leptin is about 85% homologous with human Leptin. Purified human Leptin has been shown to be biologically active in reducing body weight, food consumption, and plasma glucose levels in ob/ob and NZO mice. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 (10-100ng of Leptin protein/well) Western Blot: 1:1000-1:5000 (chemiluminescence) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice