Lck Interacting Molecule (Lck-interacting Molecule, LIME, LIME1, Lck Interacting Transmembrane Adaptor 1, Lck-interacting Transmembrane Adapter 1, Lck-interacting Membrane Protein, LP8067)

Size

£741.00

SKU:: L1566-10
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Bacterially expressed intracellular fragment corresponding to aa141-295 of human LIME.
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.