L1565-02
Lck (Lymphocyte-specific Tyrosine Kinase)
Size
£581.00
- SKU:
- L1565-02
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding the C-terminus of human Lck.
- Format:
- Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Lck, a member of the Src family of protein tyrosine kinases, is essential for T-lymphocyte activation and differentiation. It is associated with a variety of cell surface receptors and plays a key role in T-cell antigen receptor linked signal transduction pathways. The activity of Lck is regulated by protein kinases and phosphatases. Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity. The protein tyrosine phosphatase SHP-1 has been shown to dephosphorylate Lck at tyrosine 394. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice