L1565-01
Lck, p56 (Lymphocyte-specific Tyrosine Kinase)
Size
£863.00
- SKU:
- L1565-01
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-225 of mouse p56lck protein. Cellular Localization: Cell membrane
- Clone:
- 0.N.385
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See L1565-01X.
- Extra Details:
- p56lck is a membrane-associated non-receptor protein tyrosine kinase from the Src family of kinases. The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase. p56lck plays a critical role in T cell development and activation. p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Applications: Suitable for use in ELISA, Immunoprecipitation, Kinase Assay, and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: Use Protein A. 2ug antibody/mg protein lysate Western Blot: 1-2ug/ml for 2 hours at RT. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice