L1440
LAX1 (Linker for Activation of X Cells)
Size
£882.00
- SKU:
- L1440
- Purity:
- Purified by affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to aa136-398 from human LAX1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- LAX1 (Linker for activation of X cells) is a 70kD member of a small group of T cell transmembrane (TM) adaptor proteins. It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling. Human LAX1 is 398 amino acids (aa) in length. Although its predicted MW is 44kD, it runs anomalously at 70kD in SDS-PAGE. It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59-398). There are two potential isoform variants. One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1-29. Over aa 136-398, human LAX1 is 47% aa identical to mouse LAX1. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1-5x10e5 cells in a total reaction volume ≤200ul. Tested using Jurkat cells. For intraellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Direct ELISA: 0.5-1ug/ml The detection limit for rhLAX1 ~0.3ng/well. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice