La/SS-B

Size

£492.00

SKU:: L1380
Purity:: Purified by delipidation, defibrination and ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Human
Immunogen:: Purified from donor human serum.
Format:: Supplied as a lyophilized powder from PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Extra Details:: Applications: Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:100 Optimal dilutions to be determined by the researcher. Titer: Immunodiffusion: 1:8 SSB: Autoantibody Activity (by ELISA): 763EU/ml (lot-specific; value may differ from lot to lot). In an ELISA system designed to detect all IgG subclasses, the following purified antigens (dilution of 1:100) responded as: Antigen Result (EU/ml) SSA 1825 SSB 763 SCL 2.6 Sm 1.5 Sm/RNP 2.6 Jo-1 1.9 Interpretation of Results: <16 EU/ml: Negative 16-20 EU/ml: Equivocal >20 EU/ml: Positive Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.