L1350-03

Large Proteoglycan (Versican)

Size

£895.00

SKU:: L1350-03
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gly1344-Asp1554 from human Versican isoform V0, expressed in E. coli. Species Sequence Homology: canine; 80%, mouse; 60%
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Versican (versatile proteoglycan; also known as PG-M) is a 500-600kD, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa21-346), two large, alternately spliced GAG attachment domains (GAGalpha; aa348-1335; GAGbeta aa1336-3088) and a C-terminal selectin-like region (G3; aa3089-3396). Over aa1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively. Applications: Suitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml detects Versican in recombinant human Versican isoform V0. Immunocytochemistry: 5-15ug/ml detects Versican in immersion fixed HeLa cells. Immunohistochemistry: 5-15ug/ml detects Versican in immersion fixed frozen sections of mouse embroyo. Optimal dilutions should be determined by each laboratory. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice