L1227
Laminin B2
Size
£895.00
- SKU:
- L1227
- Purity:
- Purified by Protein G affinity chromatography from cell culture.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Murine EHS laminin.
- Clone:
- 0.T.83 (A5)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- Applications: Suitable for use in Immunohistochemistry, Dot Blot and Immunoprecipitation. Not recommended for Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (unfixed, frozen): 10ug/ml using monkey brain sections Dot Blot: 0.5-2ug/ml detects mouse Laminin in a dot blot assay. Optimal dilutions to be determined by the researcher. Hybridoma: Mouse-rat hybridoma of X63.Ag8 653 mouse myeloma with Fisher rat splenocytes. Immunohistochemistry 1. 10um unfixed, frozen tissue sections were washed with PBS twice, for 20 minutes. 2. Add 400ul of 8% albumin in PBS and incubate for 1 hour at room temperature. 3. Wash the sections with PBS four times, 15 minutes each, at room temperature. 4. Incubate the sections with a 10ug/ml a-Mouse Laminin B2 Chain in 1.0% albumin in PBS and incubate overnight at 4°C. Also run a negative control (no primary antibody) to check for non-specific staining. 5. Wash the sections with PBS twice for 30 minutes. 6. Incubate the sections with a 1:100 dilution of goat anti-rat IgG fluorescein conjugated secondary antibody in 1% BSA in PBS for 2 hours at room temperature. 7. Wash the section with PBS three times, 15 minutes each. 8. Examine the sections under a fluorescent microscope. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice