L1227-14-APC
Lamin B2 (APC)
Size
£914.00
- SKU:
- L1227-14-APC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Extracts from chicken nuclei(1).
- Clone:
- E-3
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Type A lamins comprise a set of three proteins arising from the same gene by alternative splicing: lamins A, C, and Adel 10. B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Mutations in A-lamins give rise to a range of genetic disorders, including autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD), dilated cardiomyopathy and Dunnigan-type familial partial lipodystrophy. Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope. Expression of A-lamin coincides with cell differentiation and may be related protein’s abnormal expression and organization in neoplastic tissues and cells. Differential lamin expression is thought to play a role in development and differentiation. Nuclear lamina in embryonic and lymphoid cells are composed exclusively of lamin B. During mitosis, lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is suggested by the fact that lamin B binds to specific DNA sequence motifs known as matrix attachment regions (MARs). MARs mediate the interaction of chromatin with the nuclear matrix. Importantly, some of the same processes which involve mitotic chromosome condensation and nuclear envelope breakdown are also activated during apoptosis. In fact, chromatin condensation paralleled by DNA fragmentation is one criterion which is used to identify apoptotic cells. Solubilization of lamin proteins has been studied in apoptopic cells, and it appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation; this degradation is useful as an apoptotic marker. Applications: Suitable for use in Immunofluorescence, FLISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 10ug/ml FLISA: 0.1–1.0ug/ml Western Blotting: 3ug/ml Immunoprecipitation: 5ug Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice