L1227-10X-BIOTIN

Laminin B2, gamma1 (BSA & Azide Free) (Biotin)

Size

£1,190.00

SKU:: L1227-10X-BIOTIN
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Murine EHS laminin preparation. Cellular Localization: Basement membrane.
Clone:: 0.N.384 (A5)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G. Immunohistochemistry (acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 min at RT. Note: Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C. Optimal dilutions to be determined by the researcher. Recommended Positive Control: LS174T cells, normal colon or colon carcinoma. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice