L1227-10
Laminin B2, gamma1
Size
£863.00
- SKU:
- L1227-10
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Murine EHS laminin preparation. Cellular Localization: Basement membrane
- Clone:
- 0.N.384 (A5)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See L1227-10X.
- Extra Details:
- Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Native and denatured): 2ug/mg protein lysate using Protein G. Immunohistochemistry (Acetone-fixed, frozen and acid-alcohol-fixed, paraffin-embedded): 2ug/ml for 30 minutes at RT. Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37°C. Optimal dilutions to be determined by the researcher. Positive Control: LS174T cells, normal colon or colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice