L1225-49B
Laminin A4 (Biotin)
Size
£895.00
- SKU:
- L1225-49B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived rmLaminin a4 (aa826-1816)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single α, β, and γ chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin α4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin α4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C-terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin α4 shows 91% and 97% aa sequence identity with human and rat Laminin α4, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse Laminin α4. The detection limit for rmLaminin α4 is approximately 10 ng/lane under non-reducing and reducing conditions. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice