L1225-46B

Laminin A1 (LAMA1)

Size

£863.00

SKU:: L1225-46B
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Purified fragment of chymotrypsin-digested mouse Laminin-1.
Clone:: AL-4
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, Laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival. Laminin-1 is comprised of alpha1, Beta1, and gamma1 subunits. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen sections): 8-25ug/ml using immersion fixed frozen sections of embryonic mouse decidua (E10.5) Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.