L1225-35-ML650
Laminin 5 (Laminin gamma 2) (MaxLight 650)
Size
£1,133.00
- SKU:
- L1225-35-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST fusion protein expressing aa382-608 of human laminin gamma 2 chain (III domain).
- Clone:
- 2Q586
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified. Applications: Suitable for use in FLISA, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested. Recommended Dilutions: Western Blot: detects Laminin 5 in 10ug of human recombinant Laminin. A previous lot detected Laminin 5, gamma 2 chain in 10ug of Human Placenta lysates. Immunohistochemistry (Paraffin): Do not over fix. A light 4% or 2% PFA is best. 4mm thick paraffin sections are dewaxed, rehydrated and immersed in 0.3% hydrogen peroxide-containing methanol for inactivation of intrinsic peroxidase, followed by treatment with Protease XXIV for 15 minutes at RT. Incubate at 37°C for 1 hour, followed by secondary antibody detection and visualize with DAB. Inhibition: 40ug/ml inhibits a2b1 integrin binding to laminin 5. Optimal dilutions to be determined by the researcher. Recommended Controls: Human pancreatic tumor tissue, human breast carcinoma tissue, human placenta lysates, A431 whole cell lysates. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice