L1225-26

Laminin

Size

£659.00

SKU:: L1225-26
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Laminin preparation from the EHS mouse tumor.
Clone:: 4A62
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Extra Details:: Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Laminin anchors cells to the basement membrane through interactions with integrins at the cell surface and collagens in the basement membrane. Applications: Suitable for use in Immunoblot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen tissue): 1:25–1:200 using avidin-biotinylated HRP complex as detection reagent. Immunoblot: 1:100-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells fused with spleen cells from a Fisher rat Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.