Laminin (Biotin)

Size

£1,081.00

SKU:: L1225-22-BIOTIN
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Laminin extracted and purified from EHC mouse tumor tissues.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Laminin is a major component of basement membranes and stimulates neurite outgrowth, promotes cell attachment, chemotaxis and cell differentation. Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. Applications: Suitable for use in Immunohistochemistry, Radioimmunoassay, ELISA and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): ≥1:500. Use Immunoperoxidase technique on fixed, paraffin-embedded mouse tissues (skin, liver) ELISA: ≥1:200 (OD ≥ 500) Immunofluorescence: ≥1:80 (Frozen mouse skin and liver tissues). Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-40D: IgG, H&L (FITC) Pab Gt x Rb I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt x Rb I1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb I1904-46N: IgG, H&L (FITC) Pab Gt x Rb Positive Control: Mouse skin or liver Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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