Laminin (Biotin)

Size

£896.00

SKU:: L1225-15
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Non reduced human laminin (400kD).
Format:: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin.
Extra Details:: Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV) and large heparan sulfate proteoglycan (HSPG). All interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. Applications: Suitable for use in ELISA, Immunoprecipitation, Immunohistochemsitry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000-1:100,000 Western Blot: 1:2000-1:10,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.