L1220-35A

Lamin B2

Size

£659.00

SKU:: L1220-35A
Purity:: Purified.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Lamin B2.
Clone:: LN43
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Extra Details:: Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blotting. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:00-1:200 Immunohistochemistry (Frozen) 1:00-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as a detecting reagent. Western Blot: 1:00-1:1000 Optimum dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.