Lamin A

Size

£581.00

SKU:: L1220-01
Purity:: Purified by Protein A and immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Asp230 of human lamin A (KLH).
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Extra Details:: Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1,3). Lamin A is cleaved by caspase-6 and serves as a marker for caspase- 6 activation. During apoptosis, the 70 kD lamin A is specifically cleaved to a large (40-45 kD) and a small (28 kD) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Flow Cytometry: 1:50 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.