L1050-61G
LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Low Endotoxin, Preservative Free)
Size
£576.00
- SKU:
- L1050-61G
- Application:
- Flow Cytometry
- Purity:
- Purified by affinity chromatography. Endotoxin: <0.01EU/ug (LAL)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Murine CD223 Ig fusion protein
- Clone:
- 7E155 (C9B7W)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added.
- Extra Details:
- CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells. Applications: Suitable for use in Flow Cytometry. Though not tested both Immunoprecipitation and ELISA have been reported in literature references. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice