L1050-61F

LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223)

Size

£550.00

SKU:: L1050-61F
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Murine CD223 Ig fusion protein
Clone:: 7E155 (C9B7W)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Murine lymphocyte activation gene-3 (LAG-3) is a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Applications: Suitable for use in Flow Cytometry and Functional Assays. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Lewis rats. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.