L1050-61D

LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Biotin)

Size

£748.00

SKU:: L1050-61D
Purity:: Purified by Protein G affinity chromatography from hybridoma tissue culture supernatant. Endotoxin: ≤0.001EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to 30aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
Clone:: 6D65 (17B4)
Format:: Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin.
Extra Details:: The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36kD) and LAG-3V3 (D1-D3, 52kD). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-gamma production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: Detection. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice