L1011-10A
Lactate Dehydrogenase A (L-lactate Dehydrogenase A Chain, LDHA, LDH-A, Cell Proliferation-inducing Gene 19 Protein, GSD11, LDH Muscle Subunit, LDHM, LDH-M, LDH1, PIG19, Renal Carcinoma Antigen NY-REN-59)
Size
£406.00
- SKU:
- L1011-10A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa1-332 from human LDHA, expressed in E. coli
- Clone:
- AT1A4
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle. Applications: Suitable for use in ELISA, Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:500 Immunofluorescence (IC): 1:500-1:8000 Western Blot: 1:500-1:3000 Optimal dilutions to be determined by the researcher. Hybridoma: FO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice