L0011-ML650
L11 Ribosomal Protein (MaxLight 650)
Size
£1,042.00
- SKU:
- L0011-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- L11-GST fusion protein
- Clone:
- 6A104
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Interactions between ribosomal protein L11 and a domain of large subunit rRNA have been highly conserved across species and are essential for efficient protein synthesis. In E. coli, L11 protein is located at the base of the L7/L12 stalk of the 50S subunit. The C-terminal domain of L11, L11-C76, recognizes and binds in the distorted minor groove of a helix within a 58-nucleotide domain of 23 S ribosomal RNA, whose secondary structure consists of three helical stems and a central junction loop. The conserved structural core of the protein consists of a bundle of three alpha-helices and a two-stranded parallel beta-sheets. When fused with His-Tag and S-tag sequences, L11 showed a molecular weight of around 21kD as expected. Recently, L11 has been described as interacting with the human homolog of MDM2, HDM2, at a region distinct from the ARF binding site. L11 binding to HDM2 inhibits HDM2 function, leading to the stabilization and activation of p53. Applications: Suitable for use in FLISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice