L0011-BIOTIN
L11 Ribosomal Protein (Biotin)
Size
£1,042.00
- SKU:
- L0011-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- L11-GST fusion protein
- Clone:
- 6A104
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- Interactions between ribosomal protein L11 and a domain of large subunit rRNA have been highly conserved across species and are essential for efficient protein synthesis. In E. coli, L11 protein is located at the base of the L7/L12 stalk of the 50S subunit. The C-terminal domain of L11, L11-C76, recognizes and binds in the distorted minor groove of a helix within a 58-nucleotide domain of 23 S ribosomal RNA, whose secondary structure consists of three helical stems and a central junction loop. The conserved structural core of the protein consists of a bundle of three alpha-helices and a two-stranded parallel beta-sheets. When fused with His-Tag and S-tag sequences, L11 showed a molecular weight of around 21kD as expected. Recently, L11 has been described as interacting with the human homolog of MDM2, HDM2, at a region distinct from the ARF binding site. L11 binding to HDM2 inhibits HDM2 function, leading to the stabilization and activation of p53. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice