L0010-06
Neural cell adhesion molecule L1 (L1CAM, L1 cell adhesion molecule, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, N-CAM L1, S10, SPG1)
Size
£863.00
- SKU:
- L0010-06
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Partially purified Neural Cell Adhesion Molecule L1 from mouse cerebellum.
- Clone:
- 9H147
- Format:
- Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- The neural cell adhesion molecule L1 (NCAM-L1/CD171), also called L1-CAM or L1, is an immunoglobulin superfamily (IgSF) adhesion molecule that is critical for neural development. NCAM-L1 binds homophilically and heterophilically with adhesion molecules including axonin-1, CD9, neurocan and integrins. Mutations of the gene encoding NCAM-L1 are associated with a subset of X-linked mental retardation syndromes, schizophrenia, and other neurological disorders. Cancer cells can proteolytically cleave NCAM-L1, releasing the soluble ectodomain. The mouse NCAM-L1 extracellular domain, aa19-1123, shares 87% and 98% aa identity with human and rat NCAM-L1, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 1x10e6 cells using mouse splenocytes Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice