L0010-01-ML490
L1 Cell Adhesion Molecule (L1CAM) (MaxLight 490)
Size
£914.00
- SKU:
- L0010-01-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 3T3 fibroblasts transfected with the human L1 protein
- Clone:
- 6A103
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The L1 adhesion molecule is a 200-220kD membrane glycoprotein of the immunoglobulin superfamily. Initially studied in the nervous system, L1 is involved in important processes such as neuron-neuron adhesion and migration, axonal outgrowth and fasciculation, myelination, and synaptic plasticity. Mutations in the L1 gene product have been shown to cause abnormal brain development. Outside the nervous system, L1 is expressed in hematopoietic and certain epithelial cells in humans. L1 is also active in the mitogen-activated protein kinase (MAPK) signaling cascade. Applications: Suitable for use in FLISA, Immunopreciptitation, Immunofluorescence and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1ug/ml Immunoprecipitation: 5-10ug/test Immunofluorescence: 5-10ug/test Immunocytochemistry: 0.5-5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human L1-transfected rat B28 cells Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice